PoplarV1, Main, Exploration, bibRecord, 002D34

Reference gene selection for quantitative real-time polymerase chain reaction in Populus.

Identifieur interne : 002D34 ( Main/Exploration ); précédent : 002D33; suivant : 002D35

Reference gene selection for quantitative real-time polymerase chain reaction in Populus.

Auteurs : Meng Xu [République populaire de Chine] ; Bo Zhang ; Xiaohua Su ; Shougong Zhang ; Minren Huang

Source :

Analytical biochemistry [ 1096-0309 ] ; 2011.

RBID : pubmed:20816740

Descripteurs français

KwdFr :
- ARN ribosomique 18S (génétique), ARN ribosomique 18S (normes), Actines (génétique), Actines (normes), Facteur-1 d'élongation de la chaîne peptidique (génétique), Facteur-1 d'élongation de la chaîne peptidique (normes), Gènes de plante (MeSH), Normes de référence (MeSH), Populus (génétique), RT-PCR (méthodes), RT-PCR (normes), Séquence nucléotidique (MeSH).
MESH :
- génétique : ARN ribosomique 18S, Actines, Facteur-1 d'élongation de la chaîne peptidique, Populus.
- méthodes : RT-PCR.
- normes : ARN ribosomique 18S, Actines, Facteur-1 d'élongation de la chaîne peptidique, RT-PCR.
- Gènes de plante, Normes de référence, Séquence nucléotidique.

English descriptors

KwdEn :
- Actins (genetics), Actins (standards), Base Sequence (MeSH), Genes, Plant (MeSH), Peptide Elongation Factor 1 (genetics), Peptide Elongation Factor 1 (standards), Populus (genetics), RNA, Ribosomal, 18S (genetics), RNA, Ribosomal, 18S (standards), Reference Standards (MeSH), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards).
MESH :
- chemical , genetics : Actins, Peptide Elongation Factor 1, RNA, Ribosomal, 18S.
- chemical , standards : Actins, Peptide Elongation Factor 1, RNA, Ribosomal, 18S.
- genetics : Populus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- Base Sequence, Genes, Plant, Reference Standards.

Abstract

Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.

DOI: 10.1016/j.ab.2010.08.044
PubMed: 20816740

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="Xu, Meng" sort="Xu, Meng" uniqKey="Xu M" first="Meng" last="Xu">Meng Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation>
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<author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name>
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<author><name sortKey="Su, Xiaohua" sort="Su, Xiaohua" uniqKey="Su X" first="Xiaohua" last="Su">Xiaohua Su</name>
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<author><name sortKey="Zhang, Shougong" sort="Zhang, Shougong" uniqKey="Zhang S" first="Shougong" last="Zhang">Shougong Zhang</name>
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<author><name sortKey="Huang, Minren" sort="Huang, Minren" uniqKey="Huang M" first="Minren" last="Huang">Minren Huang</name>
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<author><name sortKey="Zhang, Shougong" sort="Zhang, Shougong" uniqKey="Zhang S" first="Shougong" last="Zhang">Shougong Zhang</name>
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<series><title level="j">Analytical biochemistry</title>
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<term>Base Sequence (MeSH)</term>
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<term>Peptide Elongation Factor 1 (genetics)</term>
<term>Peptide Elongation Factor 1 (standards)</term>
<term>Populus (genetics)</term>
<term>RNA, Ribosomal, 18S (genetics)</term>
<term>RNA, Ribosomal, 18S (standards)</term>
<term>Reference Standards (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 18S (génétique)</term>
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<term>Actines (génétique)</term>
<term>Actines (normes)</term>
<term>Facteur-1 d'élongation de la chaîne peptidique (génétique)</term>
<term>Facteur-1 d'élongation de la chaîne peptidique (normes)</term>
<term>Gènes de plante (MeSH)</term>
<term>Normes de référence (MeSH)</term>
<term>Populus (génétique)</term>
<term>RT-PCR (méthodes)</term>
<term>RT-PCR (normes)</term>
<term>Séquence nucléotidique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Actins</term>
<term>Peptide Elongation Factor 1</term>
<term>RNA, Ribosomal, 18S</term>
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<term>Actines</term>
<term>Facteur-1 d'élongation de la chaîne peptidique</term>
<term>Populus</term>
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<term>Reference Standards</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term>
<term>Normes de référence</term>
<term>Séquence nucléotidique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.</div>
</front>
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<Title>Analytical biochemistry</Title>
<ISOAbbreviation>Anal Biochem</ISOAbbreviation>
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<Abstract><AbstractText>Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process.</AbstractText>
<CopyrightInformation>Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.</CopyrightInformation>
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<ForeName>Meng</ForeName>
<Initials>M</Initials>
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Reference gene selection for quantitative real-time polymerase chain reaction in Populus.

Reference gene selection for quantitative real-time polymerase chain reaction in Populus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki